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6.start.stop.07.v2

6.start.stop.07.v2 - Accessory factors summary 1 DNA...

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Accessory factors summary 1. DNA polymerase can’t replicate a genome. Solution ATP? No single stranded template Helicase + The ss template is unstable SSB (RPA (euks)) - No primer Primase (+) No 3’-->5’ polymerase Replication fork Too slow and distributive SSB and sliding clamp - Sliding clamp can’t get on Clamp loader ( γ /RFC) + Lagging strand contains RNA Pol I 5’-->3’ exo, RNAseH - Lagging strand is nicked DNA ligase + Helicase introduces + supercoils Topoisomerase I I + and products tangled 2. DNA replication is fast and processive
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DNA polymerase holoenzyme QuickTimeª and a DV - PAL decompressor are needed to see this picture.
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Maturation of Okazaki fragments
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Topoisomerases control chromosome topology Catenanes/knots Relaxed/disentangled Major therapeutic target - chemotherapeutics/antibacterials Type I I topos transport one DNA through another Topos
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Starting and stopping summary 1. DNA replication is controlled at the initiation step. 2. DNA replication starts at specific sites in E. coli and yeast. 3. I n E. coli , DnaA recognizes OriC and promotes loading of the DnaB helicase by DnaC (helicase loader) 4. DnaA and DnaC reactions are coupled to ATP hydrolysis. 5. Bacterial chromosomes are circular, and termination occurs opposite OriC. 1. I n E. coli , the helicase inhibitor protein, tus, binds 7 ter DNA sites to trap the replisome at the end. 2. Eukaryotic chromosomes are linear, and the chromosome ends cannot be replicated by the replisome. 3. Telomerase extends the leading strand at the end.
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