lec.1&2.prok_trxn_apparatus

lec.1&2.prok_trxn_apparatus - Come to lectures! All...

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Come to lectures! All exam questions will be from lectures. Supplemental and updated course materials will be posted on the course web site regularly; http://mcb.berkeley.edu/courses/mcb110 Interrupt me in lecture to ask questions We discuss many important concepts and principles in the context of experiments Come to my office hours – Fridays from 3 to 5 PM Make an appointment outside office hours by email qzhou@berkeley.edu Midterm: Monday, Nov. 5 in 100 GPB from 6:30 to 8:30 PM
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Why regulation of gene expression is important? Cellular function is largely dictated by the set of macromolecules inside the cell. Different macromolecules accumulate to different levels under different growth conditions and in different cell types. Diseases can be caused by aberrant control of gene expression: too much or too little of a protein; wrong time and wrong place for a protein.
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Transcription and translation in eukaryotic cells are separated in space and time. Extensive processing of primary RNA transcripts in eukaryotic cells.
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Transcription of DNA into RNA by  RNA polymerase---an overview 1.  Requires DNA template, four ribonucleotide 5’  triphosphates, Mg +2 .   2.  De novo synthesis: does not require a primer. Low fidelity compared to DNA polymerase: errors  1/10 4 -10 5  (10 5  higher than DNA polymerase).   3.  Activity highly regulated  in vivo : at initiation,  elongation and termination.
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lec.1&2.prok_trxn_apparatus - Come to lectures! All...

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