study_questions_2 - that contradicts such model? • Which...

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MCB110 – Nogales – Spring 200 STUDY QUESTIONS II M EMBRANE T RAFFICKING Describe a pulse-chase radiography experiment? How were these experiments used to work out the biosynthetic pathway? Give an example of a mutational study in yeast that allowed the identification of proteins required in the biosynthetic pathway? What are the differences in aspect and function of the smooth and rough ER? Describe the signal sequence hypothesis for the synthesis of membrane proteins in membrane-bound ribosomes. Emphasize the importance of GTP proteins and GTP hydrolysis in this model. What is (are) the main difference(s) between the synthesis of membrane proteins and soluble proteins by ER-bound ribosomes? What is the function of glycosyltransferases? What kind of substrate do they use? How could you differentiate experimentally the different functional compartment of the Golgi by direct visualization? What is the “maturation model” for the Golgi? Do you know of a experimental result
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Unformatted text preview: that contradicts such model? • Which protein residues are involved in N-linked and O-linked glycosylation? • Describe the basic principles for how COP II proteins are involved in protein selectivity and vesiculation from the ER to the Golgi? • What is the function of GTP hydrolysis in COP II vesicles? • In which two different processes are clathrin vesicles involved? • What is the general mechanism used for vesicle targeting and the protein family involved in it? • What are the two main functions of lisosomes? • What is “receptor-mediated endocytosis? What are the type of proteins involved? • Describe the function of clathrin triskelions and adaptors in endocytosis? • Describe the process of LDL uptake by cells? What elements are recycled for consecutive runs? • What is the function of dynamin in endocytosis?...
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This note was uploaded on 03/08/2010 for the course MCB 110 taught by Professor Alber,zhou,nogales during the Fall '07 term at University of California, Berkeley.

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