CH369.lecture6 - Steady-state Steady-state enzyme kinetics...

Info iconThis preview shows pages 1–14. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Steady-state Steady-state enzyme kinetics enzyme kinetics Therapeutic Drugs Therapeutic Drugs • Efficacy – ID 50 , IC 50 • Potency – dosage • Pharmacokinetics – Drug metabolism – toxicity Which statement(s) is (are) true? A.The more catalyst present, the faster the reaction. B.As product is formed, substrate concentration decreases. C. Substrate utilization is dependent on product formation D. A and B E.A, B, and C Progress of the triose phosphate Progress of the triose phosphate isomerase reaction isomerase reaction E+S E+S ES ES E + P E + P The equations of chemical The equations of chemical kinetics kinetics Consider a Unimolecular reaction A B Rate constant [ ] [ ] d A dt v k A = - = k is the rate constant The equations of chemical The equations of chemical kinetics kinetics For a bimolecular reaction A + B C The rate constant [ ] [ ] [ ][ ] d A d B dt dt v k A B = - = - = k is a second-order rate constant. Rate equation for an enzyme- Rate equation for an enzyme- catalyzed reaction catalyzed reaction 1 2 k k E S ES E P + ←→ → +-1 The rate for product formation is [ ] 2 [ ] d P dt v k ES = = The velocity of the reaction = the rate of The velocity of the reaction = the rate of appearance of the product appearance of the product Experimental conditions for steady state kinetics a) [S] >> [E] b) [ ] d ES dt = Steady-state At steady state of a reaction,...
View Full Document

This note was uploaded on 03/09/2010 for the course CH 369 taught by Professor Kbrowning during the Spring '07 term at University of Texas.

Page1 / 62

CH369.lecture6 - Steady-state Steady-state enzyme kinetics...

This preview shows document pages 1 - 14. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online