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02_COAGULASE_111509 - Clinical Unknown[Day 2 Identification...

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Clinical Unknown [Day 2]: Identification via COAGULASE PRODUCTION LABORATORY EXERCISE #2 Rev: 3/10/10 Materials and Methods Bacterial Isolates and Media Specimens were obtained by the Flamingham Bored of Health (Flamingham MA) from food handlers (nasopharangeal swabs), animals found on the premises (oral swabs), food items, and patients (vomitis) with clear-cut diagnoses of infection. Bacteria were isolated using standard media: trypticase soy agar (TSA), phenylethyl alcohol agar, blood agar, and MacDConkey agar (Difco; Sparks,MD). Staphylocicci were tested for coagulase production (BBL Staphyloside ; Becton Dickinson Microbiological Systems, Cockysville MD) and identified using the Dade Behring Microscan PC-12 Panel ( Becton Dickinson). The strains were maintained on TSA supplemented with 1% dextrose (TSA/dex); they were subcultured weekly (grown on TSA/dex for 18hr at 37°C, checked for purity, then sealed in plastic bags and stored at 4°C). COAGULASE PRODUCTION
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02_COAGULASE_111509 - Clinical Unknown[Day 2 Identification...

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