02b_Microscan_Panel_111509 - Clinical Unknown [Another Day...

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Clinical Unknown [Another Day 2]: Rapid Multitest Panels for Identification of Bacteria and for Antibiotic Susceptibility Testing LABORATORY EXERCISE #2 Rev: 3/10/10 Materials and Methods Bacterial Isolates and Media Specimens were obtained by the Flamingham Bored of Health (Flamingham MA) from food handlers (nasopharangeal swabs), animals found on the premises (oral swabs), food items, and patients (vomitis) with clear-cut diagnoses of infection. Bacteria were isolated using standard media: trypticase soy agar (TSA), phenylethyl alcohol agar, blood agar, and MacDConkey agar (Difco; Sparks,MD). Staphylocicci were tested for coagulase production (BBL Staphyloside ; Becton Dickinson Microbiological Systems, Cockysville MD) and identified using the Microscan PC-12 Panel (Semens Healthcare Diagnostics Inc., W. Sacramento CA). The strains were maintained on TSA supplemented with 1% dextrose (TSA/dex); they were subcultured weekly (grown on TSA/dex for 18hr at 37°C, checked for purity, then sealed in plastic bags and stored at 4°C). For decades biochemical procedures have been used to identify the bacteria that cause human, veterinary, and plant diseases. In many clinical labs, these series of tests have been called “long sets” - a series of various media in plates or tubes, each had to be individually inoculated and “read” the next day. Today there are numerous standardized and miniaturized versions of those biochemical procedures. The various tests are assembled into panels that have been designed to make them inoculated and read by machines, therefore allowing computer analysis of the results and direct reporting of the results to the physician. The inclusion of antibiotic sensitivity tests has increased the usefulness of the panels. Hence, the data generated by a single panel will allow the identification of a bacterium (or yeast/fungus) to the level of both genus and species and
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This note was uploaded on 03/09/2010 for the course BIOL 371 taught by Professor Eugenemuller during the Fall '09 term at Framingham State College.

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02b_Microscan_Panel_111509 - Clinical Unknown [Another Day...

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