02x_EXAMPLE_DATA_Microscan_091009

02x_EXAMPLE_DATA_Microscan_091009 - MICROSCAN GRAM-NEGATIVE...

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EXAMPLE_DATA_Microscan - LAB 4 - 3/10/10 MICROSCAN GRAM - NEGATIVE DATA SHEET NAME Dr. Blowhard LAB SECTION . Date Of Panel Set Up: Yesterday Date Of Panel Reading: Today . My unkown microbe code #: 131 . MicroScan Biocode #: 4-0-0 / 6-1-5 / 4-0-4 . Based on the MicroScan Panel, my unkown is: Proteus vulgaris . (Indicate genus and species) C G P4 GLU oil RAF INU URE oil LYS oil TDA ••• CIT TAR OF/G K 4 Cl 4 256 Sx SUC RHA ADO H2S oil ARG oil ESC MAL ACE OF/B NIT 8 16 CF SOR ARA MEL IND ••• ORN oil VP ••• ONPG CET DCB oil 32 64 Fd 4 8 To 4 16 64 Tim 2/38 T/S 8 16 C 8 16 Cfm 2 2 2 2 8 2 8 8 1 4 8/4 4 4 4 4 4 16 4 16 16 2 8 8/16 Aug 8 Nxn 8 8 8 8 32 Cax 8 32 32 4 16 4 1 16 Cfz 16 Cfx 16 Crm 16 Caz LOC X 16 Am 64 Pi 64 Ti 8 Gm 32 Ak 8 Imp 2 Cp Figure 1. The 22 antimicrobial agents (and their concentrations in μ g/mL) and 27 identification media contained in the DADE MicroScan® Neg/Urine Combo Panel. Record the MIC of each antibiotic : For another series of examples, go to the last page of this document Sx CF Fd To Tim T/S C Cfm Cax Aug Nxn >16 Cfz 4 Cfx 2 Crm Caz Am Pi Ti Gm Ak Imp Cp 1
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=================================================================== 4 - GLU = GLUCOSE - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 2 - SUC = SUCROSE - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 1 - SOR = SORBITOL - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 4 ------------------------------------------------------------------------------------------------------ 4 - RAF = RAFFINOSE - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 2 - RHA = RHAMNOSE - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 1 - ARA = ARABINOSE - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 0 ----------------------------------------------------------------------------------------------------- 4 - INO = INOSITOL - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 2 - ADO = ADONITOL - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 1 - MEL = MELIBIOSE - carbohydrate fermentation - negative = red/orange / POSITIVE = YELLOW 0 =========================================================== 2
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=================================================================== 4 - URE = hydrolysis of UREA into ammonia - negative = yellow/orange / POSITIVE = MAGENTA/PINK 2 - H 2 S = hydrolysis of production of HYDROGEN SULFIDE [H 2 S] gas - negative = not black / POSITIVE = BLACK 1 - IND = metabolism of the amino acid TRYPTOPHAN into INDOL ••• add one drop of Kovac’s reagent * - negative = pale orange/yellow / POSITIVE = RED/PINK * p-dimethylamino-benzaldehyde in HCl is caustic avoid skin contact/wear gloves 6 ------------------------------------------------------------------------------------------------------ 4 - LYS = decarboxylation of the amino acid LYSINE - negative = colorless/yellow / POSITIVE = PURPLE TO GRAY
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02x_EXAMPLE_DATA_Microscan_091009 - MICROSCAN GRAM-NEGATIVE...

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