05z_Bradford Protein Assay_BSAStdCurve_111308

05z_Bradford Protein Assay_BSAStdCurve_111308 - BSA Std...

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BSA Std Curve (diln of 1 mg/ml) mg protein A595 0.01 0.13 0.01 0.26 0.02 0.35 0.02 0.45 0.03 0.53 0.03 0.62 slope 0.02 y int 0.05 Specimen A595 mcg Protein Sample (uL) mcg Protein/mL 175 G1 0.22 9.0 10 900 175 G2 0.13 4.3 50 85 175 X1 0.22 9.0 10 900 175 X2 0.19 7.4 50 148 55 G1 0.16 5.8 50 117 55 G2 0.17 6.4 50 127 55 X1 0.12 3.7 10 374 55 X2 0.12 3.7 10 374 10 G1 0.14 4.8 50 96 10 G2 0.32 14.3 100 143 10 X1 0.27 11.6 40 291 10 X2 0.43 20.1 100 201 y=slope * x + intercept y - intercept = slope * x (y - intercept) / slope = x predicted x predicted x 0 0.01 0. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 f(x) = 19.34x + 0.0 A595
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ug protein A595 5 0.13 10 0.26 15 0.35 20 0.45 25 0.53 30 0.62 slope 0.019 intercept 0.049 x at y=0.25 10.391 x at y=0.10 2.637 .01 0.02 0.02 0.03 0.03 0.04 05
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Unformatted text preview: BSA Std Curve mg of protein mg protein A595 0.01 0.13 0.01 0.26 0.02 0.35 0.02 0.45 0.03 0.53 0.03 0.62 slope 19.34 y int 0.05 With dilution factors 175 G1 0.22 0.01 0.09 175 G2 0.13 0.01 175 X1 0.22 0.01 0.09 175 X2 0.19 0.01 0.01 55 G1 0.16 0.01 0.01 55 G2 0.17 0.01 0.01 55 X1 0.12 0.04 55 X2 0.12 0.04 10 G1 0.14 0.01 10 G2 0.32 0.01 0.01 10 X1 0.27 0.01 0.02 10 X2 0.43 0.02 0.02 0.01 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 f(x) = 19.34x + 0.05 A595 01 0.02 0.02 0.03 0.03 0.04 5 BSA Std Curve mg of protein...
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05z_Bradford Protein Assay_BSAStdCurve_111308 - BSA Std...

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