Molecular_Technologies_2_Week_9_Fall_2009

Molecular_Technologies_2_Week_9_Fall_2009 - Week 9 Notes...

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Unformatted text preview: Week 9 Notes Animal Breeding Fall 2009 Extraction of DNA can come from almost any tissue or body fluid Blood Muscle Saliva Semen Skin Concentration of DNA will vary from tissue and extraction procedure Importance of DNA extraction DNA repositories Research populations Identification of carriers of alleles through DNA and pedigree analysis Collaborative efforts of multiple researchers DNA from a foundation population and every individual born in the population in subsequent generations, or brought into the population from the outside Multi-generational analyses of inheritance Multi-generational analyses of successful selection Large population of animals to conduct marker association studies Genetic marker A detectable gene or DNA fragment used to identify alleles Microsatellites DNA which is composed of many tandem repeats of short basic repeating units Typically in non-coding regions RFLP Single Nucleotide Polymorphisms SNPs Single base pair change in DNA sequence GAACT A GCTTG GAACT G GCTTG SNP CCGTAT CACACA GTCAAC CCGTAT CACACACA CAGTCAAC Microsatellite Taking a small amount of extracted DNA and creating a larger concentration for research purposes Mimics DNA replication in a laboratory setting Forward and Reverse Primers dNTPs (nucleotides) Buffer Mg Taq Polymerase Assembles new DNA Denaturing Step Heating and unwinding (melting) of DNA Annealing Step Reaction is cooled Primers anneal to DNA DNA synthesis begins Extension/elongation New DNA synthesis via Taq begins and extension of the primer sequence Final elongation Safety step to ensure all single stranded DNA is elongated Final hold Allows for short term storage of reaction DNA C/C T/T T/T T/T T/T T/T C/C T/T T/T T/T C/T C/C T/T T/T T/T T/T T/T Ladder Utilizing up to 4 primer pairs to generate DNA fragments Can generate genotypes for four spots of interest in a single reaction...
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This note was uploaded on 03/09/2010 for the course MARB 1000 taught by Professor Hayes during the Spring '07 term at Texas A&M University-Galveston.

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Molecular_Technologies_2_Week_9_Fall_2009 - Week 9 Notes...

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