Molecular_Technologies_week_9_animal_breeding_fall_2009

Molecular_Technologies_week_9_animal_breeding_fall_2009 -...

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Unformatted text preview: Animal Breeding and Genetics Fall 2009 Definition Using genetic material (DNA) to identify an individual or determine if an individual carries and transmits a specific form of a gene Parentage testing MAS A laboratory method for characterizing an individuals DNA creating a unique genetic fingerprint Parentage testing Used extensively in animal agriculture Beef sires ? Technical definition The use of restriction fragment length polymorphism (RFLP) DNA analysis to identify an individual Having at least two forms of a gene or an allele in a population AA, Aa, aa Recognized by a restriction enzyme Enzymes with the ability to cleave (cut) double stranded DNA Restriction Fragment Length Polymorphism Inherited differences in sites for restriction enzymes that result in differences in lengths of fragments produces by clevage with relevant restriction enzyme AATTC ECOR1 DNA C/C T/T T/T T/T T/T T/T C/C T/T T/T T/T C/T C/C T/T T/T T/T T/T T/T Ladder Genetic marker A detectable gene or DNA fragment used to identify alleles Microsatellites DNA which is composed of many tandem repeats of short basic repeating units Typically in non-coding regions RFLP Single Nucleotide Polymorphisms SNPs Single base pair change in DNA sequence GAACT A GCTTG GAACT G GCTTG SNP CCGTAT CACACA GTCAAC CCGTAT CACACACA CAGTCAAC Microsatellite Genomics is the study of an organisms...
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This note was uploaded on 03/09/2010 for the course MARB 1000 taught by Professor Hayes during the Spring '07 term at Texas A&M University-Galveston.

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Molecular_Technologies_week_9_animal_breeding_fall_2009 -...

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