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Molecular_Technologies_week_9_animal_breeding_fall_2009

Molecular_Technologies_week_9_animal_breeding_fall_2009 -...

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Animal Breeding and Genetics Fall 2009
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Definition Using genetic material (DNA) to identify an  individual or determine if an individual carries  and transmits a specific form of a gene Parentage testing MAS
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A laboratory method for characterizing an  individuals DNA creating a unique genetic  fingerprint Parentage testing Used extensively in animal agriculture Beef sires 
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?
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Technical definition The use of restriction fragment length  polymorphism (RFLP) DNA analysis to identify  an individual
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Having at least two forms of  a gene or an  allele in a population AA, Aa, aa Recognized by a restriction enzyme Enzymes with the ability to cleave (cut) double  stranded DNA
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Restriction Fragment Length  Polymorphism Inherited differences in sites for restriction  enzymes that result in differences in  lengths of fragments produces by clevage  with relevant restriction enzyme
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AATTC ECOR1
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DNA C/C T/T T/T T/T T/T T/T C/C T/T T/T T/T C/T C/C T/T T/T T/T T/T T/T Ladder
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Genetic marker  A detectable gene or DNA fragment used to  identify alleles
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Microsatellites DNA which is composed of many tandem  repeats of short basic repeating units Typically in non-coding regions RFLP Single Nucleotide Polymorphisms SNP’s Single base pair change in DNA sequence
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GAACT A GCTTG GAACT G GCTTG SNP
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CCGTAT CACACA GTCAAC CCGTAT CACACACA CAGTCAAC Microsatellite
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Genomics is the study of an organisms 
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