Biology 380 Spring 2010 Immunology Henson Lec 11: Antibody Genetic Diversity I. Susumu Tonegawa’s critical experiment (he received the Nobel Prize in 1987). Questions for Hozumi and Tonegawa, 1976, Proc. Natl. Acad. Sci. USA 73:3628: 1) At the time of this paper, was it suggested that there might be multiple V region genes? Was it clear whether/ how these genes might come together to make a single protein? What were the possible explainations. 2) How were they determining the presence of the V and C regions in the restriction enzyme digested DNA? What was the purpose of using both the whole and 3'-half RNA probes in Figure 2? 3) How did they determine that the V and C gene regions in the DNA were relatively far removed in the sequence of the embryonic cell DNA and relatively close together in the DNA sequence of the plasmacytoma tumor (B) cells? 4) In Figure 4 they present some models for how DNA rearrangements could take place to produce their observed results. Explain how they support or refute the various models. 5) Can the results of this paper explain the fact that an IgM Ab and an IgG Ab can chare the exact same amino
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