Questions for Fuchs et al

Questions for Fuchs et al - What are the differences seen...

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Questions for Fuchs et al., 2007 J Cell Biol paper 1) In Fig 1 how are they determining when cell membrane integrity is compromised? Why are they using fluorescent Annexin V and is this indicating that the neutrophils are undergoing apoptosis? What are Fab fragments and why don’t they label the histone/DNA complexes in the intact cells at the start of the video? 2) In Fig 2 what is PMA and what enzyme target does it turn on that then mediates neutrophil activation? What is euchromatin and heterochromatin and how does their relative distribution change over the time of treatment? Why are they staining for neutrophil elastase and how is this being accomplished? 3) In Fig 4 how do anti-Fas antibodies induce apoptosis?
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Unformatted text preview: What are the differences seen in neutrophils undergoing apoptosis versus necrosis versus NET generation? How does the TUNEL method indicate that cells are undergoing apoptosis? 4) In Fig 5 explain how the DPI treatment blocked NET formation and how treatment with GO was able to induce NETs even in DPI-treated neutrophils. Why was catalase and its inhibitor AT included in some of these experiments and what were the results? What are PBMCs? 5) In Fig 6 why were CGD patient neutrophils included in this study and why were NET not produced by these cells with PMA treatment but were produced with GO treatment? Explain the method used to measure ROS production shown in Fig 5i....
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