ExamMULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1)Which of the following terms accurately describes the replication of DNAin vivo?1) A)nonreciprocalB)nonlinearC)dispersiveD)semidiscontinuousE)conservative 2)InE. coli, which terms accurately reflect the nature of replication of the chromosome?2) 3)DNA polymerase III adds nucleotides3) 4)DNA polymerase I is thought to add nucleotides4) 5)Structures located at the ends of eukaryotic chromosomes are called5) A)permissive mutations.B)recessive mutations.C)centromeres.D)telomerases.E)telomeres.6)Which cluster of terms accurately reflects the nature of DNA replication in prokaryotes? 6) 1
7)The discontinuous aspect of replication of DNAin vivois caused by7) SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question.8)Assume that a culture ofE. coliwas grown for approximately 50 generations in15N(provided in the medium in the ammonium ion), which is a heavy isotope of nitrogen(14N). You extract the DNA from the culture, and it has a density of 1.723 gm/cm3(water=1.00 gm/cm3). From the literature, you determined that DNA containing only the commonform of nitrogen,14N, has a density of 1.700 gm/cm3. Bacteria from the15N culture werewashed in buffer and transferred to14N medium for one generation immediately afterwhich the DNA was extracted and its density determined.(a) What would be the expected density of the extracted DNA?(b) Taking the extracted DNA, you heated it until it completely denatured (95oC for 15minutes). What would you expect to be the density of the DNA in denatured extract?Explain your answer. For the purposes of this question, assume that DNA has the samedensity regardless of whether it is single-or double-stranded.