micro midterm

micro midterm - Spring 2010 BIOL 2051 General Microbiology...

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Spring 2010 BIOL 2051 General Microbiology Laboratory Midterm Study Guide There will be four parts to the midterm: Day 1 Section A – questions about a set of materials (tubes, plates, etc) on the bench top in front of you. Section B – questions about images you have seen through the microscope. These images will be shown to you by PowerPoint presentation. Section C – short answer questions. Day 2 Section D – you will have to do a streak plate and perform a Gram stain. You will have a total of 25 minutes to do both of these. You must use your time wisely. It is recommended that you do your streak plate while your smear for the Gram stain is air drying. It is not recommended that you do one procedure first and then the other second because you may run out of time before getting done with the second procedure. I. Stains Stain Structure/ chemical stained Primary stain Mordant Decolorizer Counterstain Result Organisms to remember Simple Gram Purple stains all cells Iodine Ethanol. Gram neg cells are decolorized Safranin paints the gram neg cells. Endospore Malachite green stains both veg cells and endospores Heat H20 removes green from veg cell but not endospore Safranin stains decolorized cells (veget cells) Acid-fast Carbol-fuchsin (red stain) stains all cells Phenol Acid alcohol decolorizes cells
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micro midterm - Spring 2010 BIOL 2051 General Microbiology...

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