Analysis of Colas Report

Analysis of Colas Report - Analysis of Colas Chemistry 101...

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Analysis of Colas Chemistry 101 Laboratory, Section 22 Instructor: Craig Stanton October 7, 2008 ABSTRACT An experiment was conducted to determine the phosphate content in different colas. The relationship between concentration and absorbance was determined by creating a calibration curve. By creating solutions with different concentrations of cola and AVM, the amount of phosphate is determined with the use of the Spectrometer 20. It was determined that Rally was the cola with the highest phosphate content with .1975 mL of phosphate. Diet Coke had the lowest phosphate content with .09354 mL of phosphate. RC was in the middle with a phosphate content of .1832 mL of phosphate. My signature indicates that this document represents my own work. Excluding shared data, the information, thoughts, and ideas are my own, except as indicated in the references.
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INTRODUCTION The purpose of this experiment was to determine the amount of phosphate in colas with the use of the Specrometer 20. The Spectrometer 20 measures the percent light absorbance and light transmittance of a solution at a certain wavelength. 1 Phosphate is believed to settle an upset stomach due to its buffering capacity. 2 Three different colas were examined in order to determine which had the highest and lowest phosphate contents. Determining the amount of phosphate in colas was done by first analyzing the relationship between the color of a compound and the wavelength of light absorbed. From the knowledge gained through that experiment, a way to relate light absorbance to the concentration of a species in a solution was determined. The end goal was to be able to use the relationships discovered between concentration of phosphate and the absorbance of light to determine the amount of phosphate in three different colas. RESULTS FIGURE 1:
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Figure 1 observes the relationships between the color of a solution and the percent of light transmittance. The wavelength is what determines the percent transmittance for each color. For instance, at the highest wavelength, red and yellow colored solutions let more light through than the green and blue solutions did. At the lowest wavelength, yellow let the least amount of light through. FIGURE 2: Figure 2 measures the percent of light transmittance of potassium permanganate at different wavelengths. It is apparent that the least amound of light is transmitted at 500 nm and there is a steady increase of light transmittance after that.
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FIGURE 3: Figure 3 shows the light absorbencies of each colored solution at different wavelengths. The higher the absorbency, the lower the percent transmittance, which is apparent if compared with Figure 1. FIGURE 4:
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Figure 4 shows the light absorbencies of potassium permanganate at different wavelengths. If compared to Figure 2, it is apparent that as light absorbency goes up, percent transmittance goes down. The highest amount of light absorbed by the potassium permanganate is around 500 nm. FIGURE 5:
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Analysis of Colas Report - Analysis of Colas Chemistry 101...

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