Experiment The relationship between and Wavelength of absorbance 1 mL of food coloring was added to 50 mL of water for 4 different colors. The percent transmittance and absorbance was measured using the Spec 20 for each of the colored solutions along with a blank solution of water and a KMnO4 solution. Each of these solutions were measured at 400 nm, 500 nm, 600 nm, and 650 nm. This determined the ideal wavelength at which to measure the absorbance of the solutions. A calibration curve was created to show results. The Relationship between Absorbance and Concentration In order to determine the relationship between absorbance and concentration solutions of Potassium Permanganate (KMnO 4) were created with different concentrations. The formula M 1 V 1 = M 2 V 2 was used to create the solutions. The solutions created had a concentration of 2.0 x 10-4 moles/liter, 3.2 x 10-4 moles/liter, 4.44x10-4 moles/liter and 5.0 x 10-4 moles/liter. Using the spec 20 each solution was measured at the wave lengths of 550nM, 350nM, and 450nM. The
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This note was uploaded on 03/10/2010 for the course CHEM 101 L taught by Professor Craigstanton during the Fall '08 term at Clemson.