ch 6 ws - BIBC 102 Chapter 6: Enzymes |1 6.4: Examples of...

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B I B C 1 0 2 C h a p t e r 6 : E n z y m e s | 1 6.4: Examples of enzymatic reactions I. The Chimotrypsin Mechanism: : Bovine pancreatic chyrmotrypsin is a protease : It is specific for peptide bonds adjacent to aromatic amino acid residues : Shows transition state stabilization, acid/base catalysis and covalent catalysis : Forms an acylenzyme intermediate Acylation phase: ester linkage formed between peptide carbonyl carbon and enzyme Deacylation: ester linkage hydrolyzed and non acetalated enzyme regenerated : Catalytic triad: hydrogen bonding between three amino acids See figure of chymotrypsin reaction mechanism II. Hexokinase : Undergoes induced fit on substrate binding : Induced fit: the active site changes shape as the substrate binds III. Enolase Reaction mechanism requires metal ions : Dimer with 436 amino acid residues : Catalyzes the reversible dehydration of 2-phosphoglycerate to phospoenolpyruvate : First, Lys345 acts as a general base catalyst, abstracting a proton from C-2 of 2-
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ch 6 ws - BIBC 102 Chapter 6: Enzymes |1 6.4: Examples of...

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