study guide for micro lab

study guide for micro lab - Study Guide Microbiology...

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Study Guide – Microbiology Practical. Microscope resolution Low power- 100x total magnification High Dry 400x total, 40x objective 1000x total magnification, 100x oil immersion objective Cyanobacteria, protozoan’s, algae IN POND WATER Unpigmented, moving microorganisms will probably be PROTOZOANS - performed with wet mound slide( drop of water, place cover slip) Unpigmented = lack of tissue specialization = single celled EUKARYONS = PROTISTS - Prositsts, belong to Kingdom Protista Protozoa = tiny animals, Subkingdom Protozoa Plantlike= protists with plant like qualities, Subkingdom Algae . *** All of the above are SINGLE-CELLED TYPES *** Protozoans, produce cysts ; which reproduce asexually (cell division) survive in drought, heat, and freezing. Ubiquity of Bacteria Bacteria: Found in normal flora of humans + animals. Soil, water systems, and deep within earths crust. Bacteria LACKS a defined nucleus, therefore is PROKARYOTIC Bacteria lack organelles (mitochondria, chloroplasts) but can carry out respiration and photosynthesis. Bacteria are surrounded by a cell wall known as PEPTIDOGYCLAN . Cyanobacteria: is the largest bacteria, some have been found large enough to be seen without a microscope (pencil eraser size) Shapes of bacteria Rods (bacilli) - motile or nonmotile Cocci (spherical) – nonmotile DO NOT HAVE FLAGELLA (organelle of motility) Spirals (curved rods) – may be motile with use of Axial Filaments ( in both ends of cell )
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May occur singly, in chains, in a tetrad (pack of four) or irregular masses. The Fungi: Yeasts and Mold Fungi are Eukaryotic (have membrane bound nucleus) Fungi are non -photosynthetic, some examples are: Slime molds, water molds, mushrooms, and puff balls. Fungi = Kingdom Myceteae FIVE MAIN CHARACTERISTICS OF KINGDOM MYCETEAE - are eukaryotic - non-photosynthetic - lack tissue differentiation - cell walls of chitin / polysaccharides - propagate by spore (sexual/asexual) Differences between molds and yeasts Molds are HYPHAE (intertwining filaments) Septate – crosswalk that separates into compartments (little squares) Y shape Nonseptate- no cross walk compartments, still Y shape Molds also have asexual/ sexual reproduction done through SPORES conidia Yeats DO NOT have a true hyphae They DO have multicellular structures called pseudohyphae YEATS STUDY!!!! Example: Saccharomyces cerevisiae; for bread making and alcohol fermentation -Broth cultures - Wet mount slide - Molds : mold culture directly on slide, with lactophenol cotton BLUE stain…. agar!!!! Slide culture: MOLDS
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study guide for micro lab - Study Guide Microbiology...

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