Exam 2 Fall 2008vB

Exam 2 Fall 2008vB - Principles of Cell Biology Exam 2 Fall...

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Principles of Cell Biology Exam 2 Fall 2008 October 30, 2008 Directions: There are 75 questions in this exam with only one correct answer. All answers are to be placed on the grid sheet. Acronyms are as follows: TGN – Trans Golgi Network; RER – Rough endoplasmic reticulum; SER – Smooth endoplasmic reticulum; GPCRs – G protein coupled receptors; SRP – Signal recognition particle; RTK – Receptor tyrosine kinase. Good luck! 1. Langmuir is most associated with a. Receptor tyrosine kinases b. GPCRs c. Gap junctions d. Cell membrane structure e. Trans Golgi Network 2. GMP-PNP was used to investigate RER protein synthesis. To what component in this system does it bind which in turn will stop the release and recycling of the SRP? a. Carboxy end of the nascent protein b. SRP receptor c. Translocon d. Signal sequence e. Ribosome 3. Hydropathy plots measure or chart the hydrophobicity/hydrophilicity of a. Amino acids b. Nucleic acids c. Carbohydrates such as glucose d. Phospholipids e. All of the above 4. The SRP binds to a. The larger ribosomal subunit b. The smaller ribosome subunit c. The signal sequence d. The mRNA e. All of the above 5. Cell membranes consist of a phospholipid a. Monolayer b. Bilayer c. Trilayer d. Pentalayer e. Only Dave Matthews knows for sure 6. Which of the following facilitates the folding of proteins? a. Calzones b. Captain Carlos c. Chaperonins d. Caveolae e. Calmodulin
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7. 8-azido-cAM 32 P is a tool that can be used to study the cAMP system. To what can it covalently bind? a. cAMP-dependent protein kinase b. cAMP-dependent phosphodiesterase c. Gs α unit d. Gi α unit e. Adenylyl cyclase 8. Import of nuclear proteins occurs at the nuclear pores and necessitates which of the following that binds to the nuclear localization sequence? a. SRP b. Nucleoporin c. Exportin d. Importin e. Ras 9. You investigate a cell plasma membrane protein that is a receptor for a ligand that is secreted by the thyroid. You find that the amino end of the receptor is exposed to the extracellular media. What is the classification designation of this protein? a. Type 1 b. Type 2 c. Type 3 d. Type 4 e. Type 5 10. The basic components of gap junctions that are arranged in a radial symmetry that superficially resembles a flower are called a. Compactions b. Connexons c. Importins d. Exportins e. Translocons 11. Which of the following probes or systems was instrumental in the cell fusion experiments described in class that demonstrated that proteins can move laterally in the plane of the plasma membrane? a. Antibodies b. Gold particles c. Photoaffinity agents d. Scanning electron microscopy e. Affinity chromatography 12. Freeze fracture was used by Dan Branton to show that a. Membranes are bilipid layers b. The music at a Dave Matthews concert is so loud that it can shatter ice when placed immediately in front of a speaker c. Membranes contain a number of different size proteins d. Antibodies can bind directly to integral membrane proteins e. None of the above
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13. Milstein used both a cell free system and a similar experimental system that contained
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Exam 2 Fall 2008vB - Principles of Cell Biology Exam 2 Fall...

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