MCB3023 Exam 1 - MCB3023 Colony big clone of cells Single...

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MCB3023 Colony – big clone of cells Single cell is too small to study so we culture and multiple the microbe to better study the characteristics. Methods The first thing we need in this method is sterilization/disinfection. Culturing microorganisms Liquid – solid and semi-solid culture media Aerobic and Anaerobic cultures In vivo inoculation – before life 1. Sterilization – 100% elimination of life 2. Disinfection – reduce the number of microorganisms to a safe level (alcohols, aldehydes, oxidizing agents (h2o2), phenolic compounds) 3. Pasteurization – reduce number of bacteria that produce lactic acid in food. This allows for longer preservation of food. (milk and beer) The 5 I’s of Culturing Microbes 1. Inoculation – introduction of a sample into a container of media to produce a culture of observable growth. 2. Isolation – separating one species from another. If an individual bacterial call is separated from other cells and has space on a nutrient surface, it will grow into a mound of cells – a colony. A colony consists of one species. Techniques include – streak plate. 3. Incubation – under conditions that allow growth 4. Inspection 5. Identification Required nutrients for growth: water, carbohydrates, nitrogen, sulfur, phosphorus, and oxygen. Media – Providing nutrients in the laboratory. Media can be classified according to three properties: 1. Physical state – liquid, semisolid, and solid 2. Chemical composition – synthetic (chemically defined) and non-synthetic media (complex) 3. Functional type – general purpose, enriched, selective, differential, anaerobic, transport, assay, enumeration.
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Selective media (ie high salt concentration) – contains one or more agents that inhibit growth of one bug and encourage the growth of the desired bug. Differential media (ie pH indicator) – allows growth of several types of bugs and displays visible
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MCB3023 Exam 1 - MCB3023 Colony big clone of cells Single...

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