BICD 100 - July 2 2009 BICD 100 Lecture 2 MIDTERM ANSWERS...

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Unformatted text preview: July 2, 2009 BICD 100 Lecture 2 MIDTERM ANSWERS: ________________ Eukaryotic Genome -most is noncoding -2% human genome encodes functional genes-Satellite DNA: highly repetitive, short sequences in heterochromatin ( condensed) flanking centromeres Telomeres: short tandem repeats, gets tricky with replications [tend to lose repeats at every replication] ** In germ cells there are enzymes called telomerase: faithfully replicated telomeres immortal cell lines (cancerous) -Mini micro-satellites AGAGAG--->4Copies http://www.accessexcellence.org/RC/VL/GG/possible.php pseudogenes- evolved to point where they are no longer functional, some homology DNA Replication: Watson and Crick: Test Model::::: Possibilities: how is DNA replicated ? *******MODELS****************** Semiconservative one parent strand one newly replicated strand Conservative Dispersive each newly synthesized strand is a mix of some old and some new 1958- meselson & stahl Nitrogen-15//growing E.coli on heavy N density centrifugation heavy DNA ( all Nitrogen-15) light DNA (all NIght-14) medium DNA (mix of Nitrogen-14 , Nitrogen -15) Density Centrifugation first generation second generation semiconservative 1 medium 1 light band and 1 medium band conservative 1 heavy 1 light 1 heavy 1 light dispersive 1 medium 1 medium-light DNA Polymerase 1957 need DNA template, aNTPs nucleoside triphosphate Theta Replication...
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This note was uploaded on 03/13/2010 for the course BICD bicd 100 taught by Professor Soowal during the Spring '08 term at UCSD.

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BICD 100 - July 2 2009 BICD 100 Lecture 2 MIDTERM ANSWERS...

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