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Lecture_22_2perpage - Eukaryotic Gene Regulation Continued...

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Eukaryotic Gene Regulation Continued
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Objectives Today • Brief Review of Eukaryotic Gene Regulation • Understand how researchers study gene regulation – Reporter genes – DNA constructs • Understand 3 examples of eukaryotic regulation – Yeast GAL system – Myc-Max – Fly sex determination
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Brief Review • 3 RNA polymerases in Eukaryotes – but many similarities with prokaryotes • Cis and trans acting factors affect transcription – Enhancers/Promotors/LCR (cis) – Basal factors/Activators/Repressors (trans) • Some means of post-transcriptional regulation – RNA stability/UTR’s/Modified proteins
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Studying Gene Regulation with Reporter Genes • Reporter Gene = Gene whose expression is easy to detect • LacZ (Beta-Galactosidase) – Cleaves substrate to form blue color • GFP – “Green Fluorescent Protein” is green under fluorescent light
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Reporter Gene (GFP, LacZ) Reporter Gene is regulated by DNA of Interest Regulatory region
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Lecture_22_2perpage - Eukaryotic Gene Regulation Continued...

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