biosafety quiz - BIOSAFETY 1 Viruses viral vectors bacteria...

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BIOSAFETY:  1.)Viruses, viral vectors, bacteria, transgenic plants/animals, chlamydia, rickettsiae, recombinant DNA, fungi, brain tissues from demented patients are all classified as Biohazardous materials 2.) Biological agents like anthrax, cholera and tetrodotoxin are also considered to be agents with bioterrorist potential. 3.)Blood, blood products, semen, and synovial, pericaridal, amniotic, pleural, cerebrospinal fluids and media from human tissue culture are considered to be HIGH RISK fluids. 4.)What biosafety level are these biohazardous materials? human and primate cells and tissues, adenovirus, foodbourne pathogens (salmonella, shigella, e coli 0157:H7) - BIOSAFETY LEVEL 2 5.) Infectious agents like mycobacterium tuberculosis that can present a risk by aerosol exposure, are Biosafety level 3 materials. 6.) The percutaneous transmission rates for these viruses from lowest to highest: HIV Hepatitis C, Hepatitis B 7.)Which virus causes liver cancer? Hepatitis B 8.) HIV would prevent you from fighting off an oral fungal infection so you would have thrush as a symptom? 9.) When working with Biosafety Level 2 organisms, what type of hood MUST be used? BIOSAFETY CABINET 10.) What is the minimum Personal Protective Equipment (PPE) that must be worn when working in a Biosafety Level 2 (BSL2) lab? lab coat, gloves, eye protection (safety glasses or goggles). 11.) An exposure incident that must be reported immediately includes contact by ________ or ___________ ________ __________ with ANY of these five body sites: b lood or high risk fluid 1) eye, 2) mouth, 3) non-intact skin, 4) mucus membranes, 5) needle stick 12.) Before you discard any liquid biohazardous waste down the drain in California, what are the steps you must first take? 10% bleach (1 part bleach to 9 parts liquid waste) for 30 minutes contact time 13.) If you accidentally break a flask with a biohazardous material, what steps will you take? Use absorbant material to keep from spreading, add bleach to 10% x 30 minutes (or red Z, because won't go down the drain) pick up broken glass/sharps with forceps 14.) EVERY day before you leave the lab, you MUST bleach and empty all biohazardous
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flasks and decontaminate all working surfaces. What do you use to decontaminate the bench? What do you use to decontaminate the hood? What is the last thing you do before you leave? 10% bleach 70% EtOH and flood the surface, (or wipe and then repeat with 0.5% iodine) Wash your hands! 15.) Level 1 biohazardous solid waste is put in a ________ biohazard bag but BSL 2&3 go in _______ biohazard bags. These can be autoclaved or picked up for disposal. Which ones have to be autoclaved in a Certified Medical waste autoclave? level 1: white bags, BSL 2&3=red bag, BSL 2&3 are autoclaved in a certified
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biosafety quiz - BIOSAFETY 1 Viruses viral vectors bacteria...

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