1.)Viruses, viral vectors, bacteria, transgenic plants/animals, chlamydia, rickettsiae,
recombinant DNA, fungi, brain tissues from demented patients are all classified as
Biological agents like anthrax, cholera and tetrodotoxin are also considered to be agents
3.)Blood, blood products, semen, and synovial, pericaridal, amniotic, pleural, cerebrospinal
fluids and media from human tissue culture are considered to be
4.)What biosafety level are these biohazardous materials? human and primate cells and
tissues, adenovirus, foodbourne pathogens (salmonella, shigella, e coli 0157:H7)
BIOSAFETY LEVEL 2
5.) Infectious agents like mycobacterium tuberculosis that can present a risk by aerosol
Biosafety level 3
6.) The percutaneous transmission rates for these viruses from lowest to highest:
Hepatitis C, Hepatitis B
7.)Which virus causes liver cancer?
would prevent you from fighting off an oral fungal infection so you would have
thrush as a symptom?
When working with Biosafety Level 2 organisms, what type of hood MUST be used?
What is the minimum Personal Protective Equipment (PPE) that must be worn
when working in a Biosafety Level 2 (BSL2) lab?
lab coat, gloves, eye protection
(safety glasses or goggles).
An exposure incident that must be reported immediately includes contact by
________ or ___________ ________ __________ with ANY of these five body sites:
lood or high risk fluid 1) eye, 2) mouth, 3) non-intact skin, 4) mucus
membranes, 5) needle stick
12.) Before you discard any liquid biohazardous waste down the drain in California, what are
the steps you must first take?
10% bleach (1 part bleach to 9 parts liquid waste) for 30 minutes contact time
13.) If you accidentally break a flask with a biohazardous material, what steps will you take?
Use absorbant material to keep from spreading, add bleach to 10% x 30 minutes (or
red Z, because won't go down the drain) pick up broken glass/sharps with forceps
14.) EVERY day before you leave the lab, you MUST bleach and empty all biohazardous