Exam1_2007_and_key

Exam1_2007_and_key - Name: _ Net ID: _ Section: _ EXAM I...

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Name: ____________________________ Net ID: __________________ Section: _______ EXAM I BIOCHEMISTRY 462B - SPRING 2007 Write your name and UA Net ID on ALL pages! The Exam has 10 questions on 6 pages (including this face page) and a total of 100 POINTS. Read all questions carefully Show your work on mathematical problems, including units Answer briefly in the space provided Explain all answers (Filling all available space is NOT necessary for full credit!). The degree of difficulty tends to increase as question number goes up. Useful Constants: Faraday Constant (F or ± ) = 96,480 J / V • mol Gas Constant (R) = 8.315 J / mol • K Page 1 of 6
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Name: ________________________ Net ID: ______________________ Section: ________ Page 2 of 6 Question 1. (8 points) Describe the difference between mass action ratio Q and equilibrium constant K eq . How are they related to each other mathematically through free energy change? Question 2. (12 points) What are the effects of the following events on the rates of glycogen synthesis and glycogen degradation? Describe the effects as increase, decrease, or no effect. G l y c o g e n G l y c o g e n Synthesis Degradation (A) Increase in intracellular Ca 2+ levels (B) Increase in circulating insulin (C) Decrease in blood glucose levels (D) Increase in circulating epinephrine (E) Increase in cAMP levels ( F ) I n c r e a s e i n A M P l e v e l s Question 3. (8 points) What are the regulatory enzymes (or steps) in glycolysis? What is the common trait that sets apart the reactions they catalyze from the other reactions in glycolysis?
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Name: ________________________ Net ID: ______________________ Section: ________ Page 3 of 6 Question 4. (8 points) Hydrolysis of ATP to ADP and inorganic phosphate has a ± G ´º of –30.5 kJ/mol. List and briefly describe three of the four chemical rationales for this large negative ± G ´º. Why is ATP kinetically stable at pH 7 despite the large decrease in free energy associated with its hydrolysis? Question 5. (6 points) Thiamine pyrophosphate (TPP) is a co-factor for decarboxylation reactions. Describe two reactions (i.e. by enzyme name or reactants and products) that employ this co-factor and the consequences as a result of lack of thiamine (vitamin B1). Describe why TPP is a useful molecule to cleave stable carbon-carbon bonds.
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Name: ________________________ Net ID: ______________________ Section: ________ Page 4 of 6 Question 6. (8 points) You have just isolated a strain of bacteria from the Pacific Ocean at a depth where oxygen concentration is close to zero. During the course of examining the metabolism of this new strain of bacteria, you did not observe any production of 14 CO 2 when you incubated the bacteria with 1- 14 C-glucose. However, you did observe 14 CO 2 when you performed incubation using 3- 14 C-glucose. Given these facts, name the pathways these bacteria use to metabolize glucose. Question 7.
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Exam1_2007_and_key - Name: _ Net ID: _ Section: _ EXAM I...

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