NPB 100 Lecture 1

NPB 100 Lecture 1 - ii Staining 10 Slide 1 a Methylene blue...

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Lecture 1 1) Neurons a) Post-mitotic, don’t divide b) Integrate and transmit information c) Organized into nervous systems 2) Nervous system a) Pattern of complexity is precise b) Function: organize animal’s behavior 3) Neurons work by a) Taking in information b) Transforming it (integration) c) Conduct information d) Transmit to other neurons or targets 4) Bipolar neurons a) Dendrites i) Reception of information b) Cell body (soma) c) Axon d) Bipolar: 2 poles that come off cell body
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e) Shapes simply different, functionally similar 5) Cell receive info in a) Form of ionic currents b) Currents integrated into structure of cell i) Form a change in membrane potential 6) Transmission of information a) Requires synapses i) Transmit information selectively (1) To particular targets 7) Action Potential (spikes) 8) Shape of neurons a) Critical for function b) Monopolar i) Nucleus and single process
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c) Multipolar 9) 3 aspects of anatomy a) Development of i) Light microscopy
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Unformatted text preview: ii) Staining 10) Slide 1 a) Methylene blue i) Some neurons selectively concentrate Methylene blue 11) Golgi staining a) Heavy metal salts i) Used for preservation b) Slide 2, 3 c) Cajal mapped out input/output regions & connections d) At light microscope level i) Limited resolution 12) Electron microscopy 13) Immuno-labeling a) Use fluorescent antibodies b) Can stain cells with particular phenotypes 14) Neurons occur in epithelia a) In clusters called ganglia b) In brain i) Clusters of ganglia pulled together c) Nematodes bilateral symmetrical i) C. elegans (1) Well-defined nervous system ii) Unsegmented d) Mollusc i) Can learn ii) Serotogenic amines (1) First worked out here e) Crayfish (arthropod) i) Repetition of units in nervous system f) Vertebrates i) Brain, spinal cord ii) Nerves out to muscle iii) Pattern of segmentation...
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