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NPB 101L Complete Comprehensive Final Review

NPB 101L Complete Comprehensive Final Review - NPB 101L...

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NPB 101L Final Exam Review Lab 1 – Introductory Lab 1. Describe basic setup a. What was manipulated? i. Stimulus voltage, stimulus duration, b. What was measured? i. Waveform amplitude, waveform frequency c. What concepts were investigated? i. Accuracy in measurement, recording modes, frequency, amplitude, period 2. Effects of: a. Altering duration i. Changes frequency ii. Width of waveform is longer b. Altering voltage i. Changes the amplitude 3. Calculating: a. Amplitude i. Use p-p b. Period i. Delta T c. Frequency – units = pps/Hz i. Use the measurement tool “freq” or 1/T 4. Draw a graph for lab 1 that represents data: label x and y axes, was signal calibrated? a. X axis time (seconds) b. Y axis stimulus intensity (voltage) 5. General definitions/concepts a. Hertz i. 1/sec is a unit of frequency Time – x axis. Measured Parameter (volts, force, rate) – y axis. Lab 2 – Skeletal Muscle 1. Describe basic setup a. What was manipulated? i. Stimulus intensity, stimulus frequency b. What was measured? i. Magnitude of twitch tension c. What concepts were investigated? i. Basic skeletal muscle function ii. how nerve affects muscle activity iii. tetany iv. direct muscle stimulation d. What was investigated? i. Effects of stimulus frequency and intensity on contraction ii. Effects of Ach blocker iii. Effects of direct muscle stimulation 2. Expected results a. Increasing amplitude i. Increasing amplitude of twitch tension ii. Spatial summation b. Increasing frequency i. Increased twitch response higher than just increasing intensity alone ii. Temporal summation iii. Tetanus c. Tubocurare i. Decreased twitch tension 1
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NPB 101L Final Exam Review d. Direct stimulation i. Require greater voltage to reach same twitch tension 3. Physiology and general concepts a. Action potentials b. Cross-bridge formation c. Motor unit recruitment d. Tetany e. Sarcoplasmic reticulum f. Competitive inhibitor g. Summation (spatial and temporal) What is a motor unit? Motor neuron and all the muscle fiber it innervates Define the ‘all or none’ response for motor neurons and muscle cells. Motor neurons fire or don’t fire, no such thing as firing a partially strong action potential. AP = equal in strength each time. Muscle fiber contracts or is relaxed and when it does contract, it doesn’t contract stronger. To get a stronger contraction, more muscle fibers are recruited rather than muscle fiber contracting stronger. Thus, twitches are graded in size due to motor recruitment (spatial summation). Was the maximum tension at high frequencies greater than the maximum twitch tension? Yes, after you get maximum spatial summation, the muscle can summate temporally. Did the twitches return toward the end of the tubocurare experiment? If so, why did that occur? Has a higher affinity for tubocurare, doesn’t bind that long, but doesn’t really metabolize.
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