BIS104 Edwards F06 MT1

BIS104 Edwards F06 MT1 - BIS 104-001 Fall 2006...

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BIS 104-001 Name ____________________________________ Fall, 2006 (last) (first) Exam # _____ Midterm Exam I Instructions : 1. Print your name at the top of each page. 2. This exam consists of 4 questions on 6 pages worth a total of 60 points. Point values are given in parentheses. Count your pages to be sure that you have a complete exam. 3. Write your answers to all questions in the spaces provided. 4. Write clearly; illegible answers will receive a score of zero. Cover sheet: do not remove My signature affirms that I have complied with the requirements of the Davis Honor Code. Signature Page 1 Name ________________________
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The diagrams below depict two cells that line the airway of the respiratory system. The cell on the left (N) is from a normal individual , the one on the right (CF) is from a patient with cystic fibrosis. Information describing some of the cell components is provided below the diagrams. Questions 1a – e are based on these two cells and their components. is the CFTR protein; an integral membrane is the hypothesized mutant form protein with both internal and external of the CFTR protein. It accumulates domains and a mol wt of 210,000 daltons. in the cytoplasm and has a mol wt of 190,000 daltons . is an external peripheral protein with a mol same as normal wt of 25,000 daltons is a lipid anchored (inner leaflet) protein with same as normal mol wt of 95,000 daltons Obviously, there would be many different kinds of proteins in a real cell. But, for the purpose of these questions, consider only these three. Page 2 Name ________________________
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1. You hypothesize that the CF cell has defective chloride efflux because the mutation codes for a CFTR protein that is missing a 20,000 dalton domain that is required for proper CFTR integration into the plasma membrane. Therefore, a smaller, non-functional version of the CFTR protein accumulates in the cytoplasm of CF cells. In order to test your hypothesis you begin by analyzing a total protein extract of both cell types by SDS- PAGE. a. Sketch in and label the banding pattern you would expect to obtain if your hypothesis is correct. Total protein extract of normal cells will yield 3 separate bands when the gel is stained with Coomassie Blue. CFRT will be near the top (210 kd), the lipid anchored protein will be near the middle (95 kd), the peripheral protein near the bottom (25 kd). Total protein extract of CF cells will give the same pattern
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This note was uploaded on 03/15/2010 for the course BIS BIS 104 taught by Professor Privalsky during the Fall '09 term at UC Davis.

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BIS104 Edwards F06 MT1 - BIS 104-001 Fall 2006...

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