bis_104_final_exam_key_fall_2008

bis_104_final_exam_key_fall_2008 - Page 1 Name _ PART I:...

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Page 1 Name ____________________________________ PART I: Techniques Fill in the blank with the name of the technique or the procedure described. (12 pts) 1 . Scanning electron microscopy (SEM)_ is the microscopic procedure most useful for viewing the results of freeze-fracture / freeze- etch on a cell system. 2. phase – contrast or DIC __________ is the microscopic technique that provides the best resolution and visibility when viewing unstained live cells. 3. transmission electron microscopy (TEM) is the technique of choice for viewing the ultra-structure of fixed and stained cells. 4. fusion heterokaryons ________________ describes a procedure that results in having two nuclei from different species enclosed within the plasma membrane of a single cell. 5. single particle tracking (SPT )_________ is the technique that uses protein labeling and time-lapse video- enhanced microscopy to monitor the movement of one protein within the PM of a live cell. 6. transfection _ ______________________ is a procedure whereby a DNA construct is induced to enter a live cell. 7. autoradiography_ ___________________ is a technique for detecting the location and distribution of isotopically labeled macromolecules in a cell or tissue section.
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8. a reporter gene _______________________ is often incorporated into DNA constructs in order to determine if and when a specific gene has been expressed at the protein level (translation has occurred). 9. SDS-PAGE ________________________ is a technique that uses an electric field to separate proteins based solely on their molecular mass. 10. western blot _______________________ is the technique used to probe for and detect proteins that have been resolved by the technique described in #9 above. 11. cell-free translation system ____________is an in vitro procedure that can be used to synthesize a protein encoded by a specific mRNA. 12. fluoresence activated cell sorting (FACS) _ is a fluorochrome-laser based system that can be used to separate out specific cell types from a mixed population of live cells. Page 2 Name ____________________________________ PART II: Identification (24 pts) For each of the items listed below, describe its i) location and composition, and ii) its function(s) A. HMG-CoA reductase i) Enzyme (protein) located in the SER of animal cells. ii) HMG-CoA reductase catalyzes the rate limiting step in the cholesterol biosynthetic pathway .
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B. Signal peptidase i) Enzyme (protein) located in the luminal membrane of the RER . ii) Cleaves the signal peptide sequence from the NH2-terminal end of proteins synthesized in the RER . C.
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This note was uploaded on 03/15/2010 for the course BIS BIS 104 taught by Professor Privalsky during the Fall '09 term at UC Davis.

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bis_104_final_exam_key_fall_2008 - Page 1 Name _ PART I:...

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