bis_104_mid_i_exam_key_b08 - BIS 104-002 Spring 2008...

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BIS 104-002 Name________________________________ Spring, 2008 (last) (first) Exam #: ______ MIDTERM EXAMINATION I Cover sheet: do not detach INSTRUCTIONS: There are 5 pages not counting the cover sheet. Print your name on each page. Write clearly in the spaces provided; illegible answers will receive a score of zero. Point values are given in parentheses; 60 points total My signature affirms that I have complied with the U C Davis Honor Code. signature Page 2 Name ______________________________
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What information does the technique of freeze-fracture replication provide regarding bio-membrane (e.g., plasma membrane) composition? (2) Reveals the presence or absence of integral (transmembrane) proteins; the abundance, relative size, and their distribution pattern within the inner and outer leaflets of the membrane . 6. What microscopic technique is used to view membrane specimens prepared by freeze-fracture replication? (2) Electron microscopy. (specifically, scanning) (SEM) 7. What two structural features of membrane lipids influence the relative fluidity of a bio-membrane? (2) a ) phospholipid fatty acid chain length: longer chains = less fluidity (greater viscosity ) b ) Degree of saturation of phospholipid fatty acid chains: less saturation (more double bonds) = greater fluidity. ( amount of cholesterol in the membrane was also accepted, although this does not answer this question about the structure of membrane lipids) 8. What influence does cholesterol have on the transition temperature of a bio-membrane? (2) Cholesterol acts as a “buffer” to broaden the transition range so a membrane can remain functional over a broader range of temps. (In general, increased cholesterol decreases membrane permeability and fluidity). Page
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bis_104_mid_i_exam_key_b08 - BIS 104-002 Spring 2008...

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