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bis_104_midterm_exam_ii_fall_07

bis_104_midterm_exam_ii_fall_07 - Page 1 Name The diagram...

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Page 1 Name ______________________________ The diagram depicts a plausible model for arteriogenesis in the embryonic mouse limb skin. Peripheral sensory nerves (PSNs) 1 and/or associated Schwann cells 2 synthesize and secrete VEGF protein 3 . VEGF acts on undifferentiated (PECAM-positive; ephrinB2-negative) endothelial cells (ECs) 4 , some of which have transmembrane receptors for VEGF 5 . In response to binding VEGF, approx. 60% of the ECs begin to express ephrinB2 6 as they differentiate into arteries 7 . As the arterial vessels mature, smooth muscle cells (SMCs) 8 begin to associate with the vessel wall. You hypothesize that VEGF receptors in undifferentiated ECs are randomly distributed in the PM and are relatively free to move laterally in the membrane. However, once they bind VEGF, the receptors tend to cluster into discrete islands and lose their lateral mobility. 1. What cellular mechanisms might account for the loss of mobility in this receptor / ligand complex? (4) a) b)
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Page 2 Name ______________________________ 2. Design an experiment using fluorescense recovery after photobleaching (FRAP) to test this hypothesis Describe the results you would expect to observe if your hypothesis proved to be correct. (10) EXPERIMENT: RESULTS: 3. The erbB3 gene codes for a receptor protein in Schwann cells that is essential for their development and survival. Describe what would happened to each of the cell types in the diagram if you were to transfect all of the Schwann cells with a DNA construct that contained a defective erbB3 gene. Answer on next page (14)
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Page 3 Name ______________________________.
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