BCH4024 - Long Lecture 52

BCH4024 - Long Lecture 52 - Lecture 55: Protein synthesis...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Protein synthesis Flow of genetic information --- the “Central Dogma” DNA RNA Protein Replication Transcription Translation Protein trafficking Lecture 55: Protein synthesis and translation Today’s lecture: Chapter 27b Download from http://lss.at.ufl.edu Chain elongation in a prokaryotic system EF Elongation Factor protein aatRNA has free amino end Peptidyl chain is ester-linked to tRNA in P site EF-Tu recycling Ester link is replaced by amide bond to amino acid on the tRNA in the A site by peptidyl transferase Protein synthesis is NH 3 + Æ COO -
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 “translocation” Chain elongation: getting ready for the next round aatRNA-EF-Tu-GTP EF-G-GTP Complex that charges the A site Complex that empties the A site aatRNA EF-Tu How EF-G-GTP moves things along in prokayotes
Background image of page 2
3 Chain termination in a prokaryotic system The release factors RF1 or RF2 bind in A site to stop codons UAA (RF1 or RF2), UAG (RF1), or UGA (RF2) RF3 and GTP bind Æ release RF1 or RF2 rRNA may be involved in termination as well Ribosome is recycled RF Release Factor protein mRNAs can be translated very quickly and in tandem
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 In prokaryotes transcription and translation can happen simultaneously Antibiotic inhibition of translation Causes premature chain termination by entering the A site and transferring to the peptidyl chain Puromycin
Background image of page 4
5
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/15/2010 for the course BCH 4024 taught by Professor Allison during the Spring '08 term at University of Florida.

Page1 / 11

BCH4024 - Long Lecture 52 - Lecture 55: Protein synthesis...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online