BCH4024 - Long Lecture 52

BCH4024 - Long Lecture 52 - Lecture 55 Protein synthesis...

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1 Protein synthesis Flow of genetic information --- the “Central Dogma” DNA RNA Protein Replication Transcription Translation Protein trafficking Lecture 55: Protein synthesis and translation Today’s lecture: Chapter 27b Download from http://lss.at.ufl.edu Chain elongation in a prokaryotic system EF Elongation Factor protein aatRNA has free amino end Peptidyl chain is ester-linked to tRNA in P site EF-Tu recycling Ester link is replaced by amide bond to amino acid on the tRNA in the A site by peptidyl transferase Protein synthesis is NH 3 + Æ COO -
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2 “translocation” Chain elongation: getting ready for the next round aatRNA-EF-Tu-GTP EF-G-GTP Complex that charges the A site Complex that empties the A site aatRNA EF-Tu How EF-G-GTP moves things along in prokayotes
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3 Chain termination in a prokaryotic system The release factors RF1 or RF2 bind in A site to stop codons UAA (RF1 or RF2), UAG (RF1), or UGA (RF2) RF3 and GTP bind Æ release RF1 or RF2 rRNA may be involved in termination as well Ribosome is recycled RF Release Factor protein mRNAs can be translated very quickly and in tandem
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4 In prokaryotes transcription and translation can happen simultaneously Antibiotic inhibition of translation Causes premature chain termination by entering the A site and transferring to the peptidyl chain Puromycin
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5 Tetracycline
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