BCH4024 - McKenna Lecture 45

BCH4024 - McKenna Lecture 45 - BCH 4024 Introduction to...

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1 BCH 4024 Introduction to Biochemistry and Molecular Biology 6 th Period Spring 2008 Molecular Biology Dr. Mavis Agbandje-McKenna (Office hours: Fridays 2-4pm, LG-181) Lectures L45 to L57 , Final Exam will be April 28 th , 7:30-9:30am Place to be announced Topics covered by Dr. Mavis Agbandje-McKenna L45 Nucleotides and Nucleic Acids Pages 273-292 L46 Biosynthesis and degradation of Pages 862-878 Nucleotides L47 Genes and Chromosomes Pages 923-943 L48 DNA Replication Pages 950-966 L49 DNA Synthesis Pages 298-299 L50 DNA damage and repair Pages 293-296 & Pages 966-978 L51 DNA Recombination Pages 978-992 Notes (pdf) available at The UF e-learning web site for BCH 4024
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2 INTRODUCTION TO NUCLEIC ACIDS 1868 - DNA was discovered by Friedrich Miescher In the nuclei of pus cells surgical bandages. 1943 - Avery, Macleod and McCarty The transforming principle - a dead pathogenic bacteria able to give its DNA to an avirulent strain, which when co-injected into a mouse > death. (FIG. 8-12) 1952 - Hershey-Chase Experiment >DNA is the genetic material By radiolabeling a bacteriophage (FIG. 8-13) -radioactive sulphur to tag protein -radioactive phosphorus to tag DNA -separate cells from supernatant after infection >>>> Radioactive phosphorus was found in the cells which generated new infectious bacteriophage. The “Central Dogma” of Biology Replication Transcription Translation Protein mRNA DNA
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3 THE CENTRAL DOGMA - Expanded ACTIVITY ENZYME RNA RNA RNA REPLICASE (VIRAL REPLICATION) RNA DNA REVERSE TRANSCRIPTASE DNA DNA DNA POLYMERASE (REPLICATION) DNA RNA RNA POLYMERASE (TRANSCRIPTION) RNA PROCESSING SPLICEOSOME (SPLICING) RNA PROTEIN RIBOSOME (TRANSLATION) DNA RNA PROTEIN RNA Replicase DNA POL RNA POL Reverse Transcriptase Phenotype Genetic Infor. INTRODUCTION TO NUCLEIC ACIDS – Building Block Monomeric units = Nucleotides 1’ Missing in DNA Replaced with H FIG. 8-1A
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4 INTRODUCTION TO NUCLEIC ACIDS – The Bases FIG. 8-1 B INTRODUCTION TO NUCLEIC ACIDS – The Bases Two Bases Three Bases Differentiates T from U FIG. 8-2 3 2 1 6 5 4 7 8 9 3 2 1 6 5 4 7 8 9 1 2 3 4 5 6 2 3 4 5 6 2 3 4 5 6 1 1
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5 INTRODUCTION TO NUCLEIC ACIDS -Nucleotides Bases Attached at N9 to Sugar at C1’ Bases Attached at N1 to Sugar at C1’ Glycosidic bond Ribonucleotides Deoxyribonucleotides FIG. 8-4 NUCLEOSIDES H Phosphate dAdenosine dAdenylic acid (dAMP) Adenine Deoxyribose Glycosidic bond Deoxyadenosine 5’-monophosphate (dAMP) dATP Deoxythmidine 5’-monophosphate (dTMP) dTTP
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This note was uploaded on 03/15/2010 for the course BCH 4024 taught by Professor Allison during the Spring '08 term at University of Florida.

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BCH4024 - McKenna Lecture 45 - BCH 4024 Introduction to...

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