BCH4024 - McKenna Lecture 55

BCH4024 - McKenna Lecture 55 - DNA SYNTHESIS Nature -...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Nature - Replication -DNA replication uses the sequences of the bases in DNA and RNA as a template. Laboratory - Synthesis By using enzymes (PCR). -Can chemically make pure oligo deoxynucleotides larger than 100 bases in DNA synthesizers - ~$1.50 per base. -These oligonucleotides can be been used for: Structural studies of DNA Gene modification Primers in DNA sequence determinations & synthesis -A common chemical process uses activated derivatives of bases - Phosphoramidites - contain trivalent phosphorus ( likes to be pentavalent ). DNA SYNTHESIS 1. Residues must be added one at a time, using conditions that ensure efficiency of the coupling during the successive reactions. 2. To avoid unwanted side reactions, all potentially reactive groups on the molecules (except those where the reaction is desired) must be chemically blocked. 3. Unlike the enzymatic process, DNA is synthesized chemically from 3’ to the 5’ direction - i.e. monomeric units are added at the 5’ end.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 5

BCH4024 - McKenna Lecture 55 - DNA SYNTHESIS Nature -...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online