BIS104 Slide18

BIS104 Slide18 - Mammals can synthesize over 1015 different...

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Mammals can synthesize over 10 15 different antibody molecules How is this vast antibody diversity generated? 1) Hundreds of different genes encode different variable regions for the L and H chains that form antibody 2) These L and H sequences are expressed independently, and both contribute to antigen binding pocket. Thus, multiple combinations of L and H chains 3) During B cell development different variable regions (DNA) are pieced together , and additional somatic mutations that complete generation of this diversity H L Variable region: binds to antigens Constant region: different classes of Ab
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Figure 25-30 Molecular Biology of the Cell (© Garland Science 2008) Constant and variable regions of immunoglobulin chains
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Figure 25-36 Molecular Biology of the Cell (© Garland Science 2008) The V-J joining process involved in making a light chain FYI: Allelic exclusion: only one copy ( either from dad or mom ) is V-J joined during B cell development
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Figure 25-36 Molecular Biology of the Cell (© Garland Science 2008) Antigen driven Somatic hypermutation
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Figure 25-37 Molecular Biology of the Cell (© Garland Science 2008) The V-D-J joining of the Heavy chain -> similar to the light chain V-J joining IgM IgD IgG IgE IgA Selection of the constant region of the heavy chain determines the “class” of the antibody VJ and VDJ joining of the light and heavy chain variable region determine the specificity of the antibody
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Class Switching Surface IgM is converted to circulating IgM by an alternative mRNA splicing event (deleting membrane anchor on C-terminus of heavy chain constant region) IgD is made by a second form of alternative mRNA splicing B cell making IgM is switched to making IgG, IgA, or IgE by DNA recombination event joining heavy chain VDJ to different C regions
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Figure 25-41 Molecular Biology of the Cell (© Garland Science 2008) IgG,IgE,IgA Irreversible changes circulating IgM IgD mRNA splicing M D G E A
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IgM During B cell development -antigen independent -antigen diversity come from VJ, VDJ sloppy joining… etc Antigen exposure Make circulating Ig M (2nd class switching) IgD Mature naïve B cell Making IgD- First class switch more class switching IgG IgA IgE Helper T cells
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This note was uploaded on 03/15/2010 for the course BIS 104 taught by Professor Scholey during the Fall '08 term at UC Davis.

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BIS104 Slide18 - Mammals can synthesize over 1015 different...

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