Lab3Analysis - February 4, 2010 Paul Gonzales BIO206L...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
February 4, 2010 Paul Gonzales BIO206L Session 49540 Lab 3 Analysis 1) Only the 100X objective requires the use of immersion oil because as you increase the objective size, the resolution and clarity of the image seen becomes less and less, so the oil serves as a medium other than air for light to travel through and increasing the resolution, or clarity of the image. The proper way to apply an oil immersion on the slide is to first find the object you want to view under typical bright field magnification at the 40X objective. Next you need to rotate the objectives and focus the image on the object at the 100X objective. Finally, you turn the nosepiece until the 40X objective is on one side and the 100X on the other side of the specimen. Place a drop of oil in between them where the beam of light is focused on your specimen and then rotate the 100X objective back onto the specimen ensuring a medium of oil between the lens and the specimen on the slide. The focal plane should never be adjusted when adding immersion oil because the microscope should already be focused on the specimen you want to view, the oil only makes the image clearer. After immersion oil is used, you must first clean the lens objective and the stage of any excess oil as well as the cover slip if you wish to view the specimen again at a lower objective. 2) Red blood cells contain chromatin and also a red pigment that is easily seen to the naked eye, and cytoplasm. White blood cells contain a nucleus, cytoplasm with granules, and a cell membrane. Surface Area of Sphere: 4* π *r 2
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
February 4, 2010 Paul Gonzales BIO206L Session 49540 Volume of a Sphere: (4/3)* π *r 3 Surface Area Volume Red Blood Cell 201.06 μ m 2 268.08 μ m 3 White Blood Cell 4536.46 μ m 2 28,730.91 μ m 3 The white blood cell is larger. Red blood cells are more prevalent than white blood cells. There are about 40 red blood cells for each white blood cell present in a sample of blood. 3) Phase contrast microscopy works by increasing the amount of
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/15/2010 for the course BIO 49540 taught by Professor Robertfarris during the Spring '10 term at University of Texas at Austin.

Page1 / 7

Lab3Analysis - February 4, 2010 Paul Gonzales BIO206L...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online