Exam II-2007 - NAME_ BIO362 EXAM II March 16, 2007 1. Name...

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1 NAME___________________________ BIO362 EXAM II March 16, 2007 1. Name two properties that reverse transcriptase and telomerase have in common. (6 pts) 2. What is the advantage of using partial digestion by a restriction enzyme that has a 4 bp recognition site for making a genomic library over using a restriction enzyme with a 6 bp recognition site? (4 pts) 3. What is the advantage of a mouse liver cDNA library over a genomic library made from mouse liver? (4 pts)
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2 4. SauIIIA and BamHI are restriction enzymes whose recognition sequences are shown below. The arrow indicates the bond cleaved. Suppose you want to insert a SauIIIA DNA fragment into the BamHI site of the circular vector, pUC18. Draw the insert DNA after it is base-paired with the cut vector, but before ligase has been added. Draw the sequences at the insert-vector junctions, and clearly indicate the nicks to be ligated. To make your diagram clear, draw the insert DNA with wiggly lines and the vector with straight lines. Below your drawing, answer the following question. After ligation of the nicks to make a new recombinant plasmid, will you be able to cut the insert out of the vector by cutting with BamHI? Briefly explain your answer. (16 pts) SauIIIA BamHI NGATCN where N is any of the 4 bases GGATCC
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Exam II-2007 - NAME_ BIO362 EXAM II March 16, 2007 1. Name...

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