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March 3 lecture - extra figures

March 3 lecture - extra figures - {A DNA pelvmereee DNA =...

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Unformatted text preview: {A} / +DNA pelvmereee DNA = SEEflRE HYBRIDIZATION +dATF’ SYNTHESIS deubIe-Strended STRANDS 0F PHIMEHS +dGTP FROM DNA ”“3“” PHIMEFIS +d'ITP — h _L# 5’ "h m STEP 1 STEP 2 STEP 3 FIRST CYCLE Figure 8—39 part 1 ef 3. Meleeuler Bielegv ef the Cell. 4th Edition. (Bl separate DNA strands and anneal 5 Emisis separate DNA DNA primer V }' strands and add . —-—— primer S‘fflthESIS / ‘F _ \ I. — *3 l—I DNA eligenueleetide I_I primers I—If—l — ——— / region a . _ deuble- stranded —/ \- ehrernesernal DNA te be ampllfied —\‘—-—= / ‘I _ FIRST CYCLE SECOND CYCLE {preduelng TWO double-stranded {producing feur double-stranded DNA molecules} DNA meleeulesl Figure 3—39 part 2 at 3. Meleeular BiDIDQ? ef the Cell, 4th Edition. eepe rate DNA etrendeend anneal DEA. primer 5?" 35's .r —__ I ‘- l _. \— _ ..--""" ‘ "‘ \ n EYE. i i i Ir i “'\ /\ I I! 1 I K _ THIRD CVCLE {producing eight double-stranded DNA m'eleeL'Iles} Figure 8—39 part 3 of 3. Molecular Elielegair of the Cell, 4th Editien. cells .05 isolate DNA I PCR AM PLIEICATION genomic clones (A) Figure 8-40 part 1 of 2. Molecular Biology:r of the Cell, 4th Edition. ...
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