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probset_4_2009 - (b hydroxylamine 5 The following...

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BMB 100A Fall 2009 Problem Set #4 Name___________________ 1. Show how tautomerization of guanine can lead to (a) a G-C to A-T mutation or to (b) an A-T to G-C mutation. 2. Phosphodiester bonds in RNA, but not DNA, are easily cleaved in the presence of NaOH. In the presence of small amounts of manganese ions, DNA polymerase can be made to incorporate ribonucleotides as well as deoxyribonucleotides. Explain how you would exploit this information to devise a new method for sequencing DNA. 3. Classify the following mutations: A-T to G-C G-C to A-T G-C to C-G G-C to T-A
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4. Which of the mutations in problem #3 would you expect to undergo high rates of reversion in the presence of (a) 5-bromouracil?
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Unformatted text preview: (b) hydroxylamine? 5. The following represents part of the parental strand of an E. coli DNA duplex containing an A-C mismatch caused by a replication error at the C indicated in boldface. Show the sequence of events used by the excision repair system (MutS, MutL, MutH, etc) to repair this error. (3’) …. .ATCCAGCATCTA*GCAAGGGCACT C GACGTCCATTGC….(5’) 6. Indicate the two most likely positions for UV-induced damage within the DNA represented by the sequence shown in problem 5. 7. List two sources of potential DNA damage resulting from going for a run in the country on a nice day....
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