ProblemSet1-1

ProblemSet1-1 - Bioc100B Winter 2010 Rubin Problem Set #1...

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Bioc100B Winter 2010 Rubin Problem Set #1 Due Tuesday, January 12 th 10:00 A.M. 1) Size-exclusion chromatography is a technique that can be used to determine approximately the molecular weight of a protein, protein complexs, or simple protein mixtures in solution. You perform size-exclusion chromatography on the same multiple- subunit protein under five different sample conditions and make the following measurements: Sample Solution Conditions Molecular Weight(s) Observed 1 Native buffer (pH 7.4, 150 mM NaCl), 20 ° C 100,000 Da 2 pH 7.4, 1M NaCl, 20 ° C 60,000 Da, 40,000 Da 3 6M Urea, 20 ° C 60,000 Da, 20,000 Da 4 6M Urea, 10 mM 2-mercaptoethanol, 20 ° C 50,000 Da, 20,000 Da, 10,000 Da 5 10 mM 2-mercaptoethanol, 65 ° C 50,000 Da, 20,000 Da, 10,000 Da a) Draw a diagram describing a plausible subunit composition for the protein. b) What does the experiment tells you about the forces that stabilize the different interfaces between the subunits within the quaternary structure? c) Explain the different thermodynamic mechanisms for subunit dissociation in samples
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This note was uploaded on 03/16/2010 for the course BIOC 100B taught by Professor Sethrubin during the Winter '10 term at UCSC.

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ProblemSet1-1 - Bioc100B Winter 2010 Rubin Problem Set #1...

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