ProblemSet3-4

ProblemSet3-4 - Bioc100B Winter 2010 Rubin Problem Set#3...

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Bioc100B Winter 2010 Rubin Problem Set #3 Due Tuesday, January 26 th 10:00 A.M. 1) Explain how each of the following observations is either consistent or inconsistent with the symmetry and sequential models of allosterism in ligand binding. a) In almost all crystal structures of hemoglobin (including both liganded and unliganded), all four subunits are in the same conformation. b) Mutations that disrupt interactions between subunits often disrupt allosteric effects. c) Crystal structures of proteins trapped in a either a T or R state (by mutagenesis for example) show structural changes upon ligand binding. d) There is experimental evidence supporting examples of negative homotropic allosterism. 2) I mentioned briefly in class that p53 is a DNA-binding transcription factor that is often mutated in cancer. Here is a proposal for an experiment to measure the dissociation rate of DNA from p53: Bind p53 quantitatively with DNA that has a radioactive probe in it (e.g. 32
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ProblemSet3-4 - Bioc100B Winter 2010 Rubin Problem Set#3...

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