ProblemSet4-2 - Bioc100B Winter 2010 Rubin Problem Set#4 Due Tuesday February 9th 10:00 A.M 1 Derive the Michaelis-Menten equation for a mixed

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Bioc100B Winter 2010 Rubin Problem Set #4 Due Tuesday, February 9 th 10:00 A.M. 1) Derive the Michaelis-Menten equation for a mixed inhibitor (an inhibitor that binds both E and ES). You can assume that substrate binding to EI is negligible. 2) Proteases catalyze the hydrolysis of peptide bonds. The HIV protease cleaves HIV gag proteins at specific sites. Propose a kinetic assay that could be used to measure the K I of the competitive HIV protease inhibitor Ritonavir. Some considerations: What are the known quantities in your enzyme catalyzed reaction? What is the data you actually measure and how do you measure it? How do you plot this data for analysis? Sketch examples. What kinetic constants do you extract from analysis of the data and how do you calculate them? How can you vary the reaction conditions (i.e. known quantities in the reaction) to manipulate the kinetic constants and derive K I? 3) Read the recent review article by James Robertson entitled “Mechanistic Basis of Enzyme-
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This note was uploaded on 03/16/2010 for the course BIOC 100B taught by Professor Sethrubin during the Winter '10 term at University of California, Santa Cruz.

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