ProblemSet5-1 - BMB100B Winter 2010 Rubin Problem Set #5...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BMB100B Winter 2010 Rubin Problem Set #5 Due Tuesday, February 19 th 10:00 A.M. 1) Another type of protease that carries out the same peptide hydolysis reaction as serine proteases is the Zn protease. Below is a schematic of the active site containing the Zn and a Glu. Propose a mechanism for the Zn protease catalyzed hydrolysis reaction. Use the picture and add a water to depict the beginning (ES complex) state and draw the tetrahedral intermediate and product states. Be sure to indicate movement of electrons with arrows and all formal charges. b) The serine protease mechanism (e.g. chymotrypsin, etc.) is often characterized as covalent catalysis. Would you characterize the zinc protease mechanism as covalent catalysis? What is the key difference between this mechanism and the serine protease mechanism? 2) Leupeptin is a common protease inhibitor. Here is its structure: Use the structure of trypsin bound to leupeptin (PDB ID: 1JRT) to answer the following: a) Explain the mechanism of leupeptin inhibition. b) Design a leupeptin analog that would inhibit chymotrypsin.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/16/2010 for the course BIOC 100B taught by Professor Sethrubin during the Winter '10 term at University of California, Santa Cruz.

Page1 / 2

ProblemSet5-1 - BMB100B Winter 2010 Rubin Problem Set #5...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online