SolutionSet4-1

SolutionSet4-1 - Bioc100B Winter 2010 Rubin Solution Set #4...

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Bioc100B Winter 2010 Rubin Solution Set #4 1) Derive the Michaelis-Menten equation for a mixed inhibitor (an inhibitor that binds both E and ES). You can assume that substrate binding to EI is negligible.
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Bioc100B Winter 2010 Rubin 2) Proteases catalyze the hydrolysis of peptide bonds. The HIV protease cleaves HIV gag proteins at specific sites. Propose a kinetic assay that could be used to measure the K I of the competitive HIV protease inhibitor Ritonavir. Some considerations: What are the known quantities in your enzyme catalyzed reaction? What is the data you actually measure and how do you measure it? How do you plot this data for analysis? Sketch examples. What kinetic constants do you extract from analysis of the data and how do you calculate them? How can you vary the reaction conditions (i.e. known quantities in the reaction) to manipulate the kinetic constants and derive K I?
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Bioc100B Winter 2010 Rubin
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Bioc100B Winter 2010 Rubin
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Bioc100B Winter 2010 Rubin 3) Read the recent review article by James Robertson entitled “Mechanistic Basis of Enzyme- Targeted Drugs” in the journal Biochemistry. The reference is as follows: Robertson, J.G. Biochemistry, Volume 44, Issue 15, pages 5561-5571, 2005. You can access the journal online and download the paper from a computer with a ucsc.edu address (for example, computers in the Science and Engineering library). a) From his survey Robertson concludes that a majority of enzyme-targeting drugs have
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SolutionSet4-1 - Bioc100B Winter 2010 Rubin Solution Set #4...

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