100cps5s09

100cps5s09 - Biochemistry Molecular Biology 100C Problem...

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Biochemistry & Molecular Biology 100C Problem Set #6 due: May 15, 2009. 1. For the following balanced electrochemical reaction, construct a plot of observed voltage (ordinate) versus equivalents of electrons transferred (abscissa): Reference cell = 1.0 M NADH Test cell = 1.0 M FAD Given: FAD + 2H + + 2e - FADH 2 E 0 = – 0.22 V NAD + + 2H + + 2e - NADH + H + E 0 = – 0.32 V Assume both cells contain one liter of solution and are buffered to retain the ‘prime’ condition, i.e. pH 7.0 at 25 C. Fill in and then use the following data points to construct your plot: e - (equivalents) [FAD] [FADH 2 ] [NAD + ] [NADH] (0) 1.0 M - - 1.0 M 0.02 0.2 0.6 1.0 1.4 1.8 1.98 (2.0) 2. When rat liver mitochondrial inner-membrane preparations are treated with the inhibitor antimycin A in the presence of excess NADH, O 2 consumption stops. (a) Using difference spectroscopy, which cytochrome (heme groups) would then be reduced, and which would be oxidized? If excess vitamin C, ascorbic acid, is then added to these inhibited membrane preparations, oxidative phosphorylation (both respiration and ATP synthesis) resumes. (b) Estimate the ~P/O ratio (ATP resynthesized per ½O 2 consumed) for O 2 sparged, buffered mitochondrial membranes with added ADP, P i , NADH and ascorbic acid in the absence of antimycin A treatment. (c) Estimate the ~P/O ratio in the presence of excess antimycin A.
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This note was uploaded on 03/16/2010 for the course BIOC 100A taught by Professor Harrynoller during the Winter '10 term at UCSC.

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100cps5s09 - Biochemistry Molecular Biology 100C Problem...

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