PS7-4Sol - with glucose column. However, you can figure...

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Name answers 12. Lac Operon (30 points) A. Given the genotypes, fill in the appropriate phenotypes for LacZ and LacY. The inducer is lactose or IPTG. Phenotypes LacZ expression LacY expression Genotype no inducer inducer no inducer inducer 1 I + O + Z + Y + 2 I S O + Z + Y - 3 I S O + Z - Y - / F’I - O c Z + Y + 4 I + O c Z - Y - / F’ I-O + Z - Y + 5 I - O C Z - Y + / F’ I S O + Z + Y - B. Name one strain that indicates that O C is epistatic to I S , and what data tells you that? Strain 5- that Y is expressed tells you that O C is epistatic to I S Strain 3: both Z+ and Y+ expression tells you that O C is epistatic to I s C. A new gene is identified that regulates the Lac operon, called Gene T. An allele Gene T-2 was analyzed. We know that Gene T+ acts in the glucose, ADC1, CRP pathway. The assays of Gene T+ function are done in a I- cell, with glucose present. (The phenotypes would be complicated by reporting LacZ expression with no glucose and
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Unformatted text preview: with glucose column. However, you can figure this out using this data alone). LacZ expression 1. GeneT+ -2. GeneT-2 + 3. GeneT-2/ Gene T+ + 4. ADC1* + 5. ADC1* GeneT-2 + 6. ADC1- -7 . ADC1- Gene T-2 + i. Is Gene T-2 recessive or dominant (circle one) ii. Draw the glucose, Gene T+,ADC1,CRP, LacZ, pathway, with gene order and sign of regulation (bar or arrow) iii. Why is the combination of strains 2, 4 and 5 not informative for trying to determine the order of function of GeneT+ and ADC1+? both single mutants have the same phenotype.--+ + 8. CRP - -9. CRP- GeneT-2 -Strain 10 pt, 1/2 ea 2pt 2pt- - - -+ + + +- - - +- - + + Glucose ADC1+ GeneT+ CRP+ LacZ...
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This homework help was uploaded on 04/03/2008 for the course BIO 320 taught by Professor Walsh,weinert during the Fall '07 term at University of Arizona- Tucson.

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