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Unformatted text preview: 12-carbon fatty acid using acetyl CoA as the starting material? How many NADPH are needed? If you use glucose as a starting material, how many ATPs are needed? How many glucose molecules are needed? You should be able to trace labeled carbon or hydrogen during fatty acid biosynthesis. For example, if you use labeled CO2, mixed with fatty acid synthase complex and acetate to make a 16-carbon fatty acid, where will the label be? If you add labeled acetyl-CoA to liver extracts, where will the label be in fatty acid (16-Carbon) If you add a trace amount acetyl CoA in the presence of large excess of malonyl-CoA, where will be the label in a 16-carbon fatty acid? If you use labeled glucose (label Carbon 3), where the label will be in fatty acids? Know the cellular locations of fatty acid catabolism and anabolism. Know how acetyl group is shuttled between mitochondria and cytosol (know the details of the shuttle intermediates)...
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- Spring '02