Unformatted text preview: Problem Set 7 Due Oct 21 1.True/False short answer.This is one kind of problem I give on exams.Answer True or
False, and provide one or two sentences that says something relevant about the question.
Your statement must be relevant to get credit for the True/False response. For some of
them you do have to think carefully about how to say something tersely, and some
responses should be very easy. A. Mendel would have discovered the same principles of genetic recombination if he had
been studying bacterial genetics. False. Bacterial do not have reciprocal genetic exchange. Bacteria are haploid so segregation of
two alelles at one locus can’t be determined. Bacteria dont’do mitosis. Any of these is good.
B.Transduction requires a single crossover to introduce a donor gene into the recipient
chromosome. False. It requires two crossovers, because one crossover of a linear by circle gives a linear which
is dead in bacteria. C.Gene transfer from a donor to recipient in an Hfr X F- cross requires that the Hfr be
recA+. False. recombination does not occur in the donor,on|y the recipient; recA required in the
recipient. D. Cyclin E overexpression might cause cancer in a way that is similar to Cyclin D
overexpression. True. cyclinD-CDK causes inhibition of Rb (see path way), so overexpression of cyclin D would be
expected to cause greater levels of inhibition of Rb. Similarly, cyclinE inhibits Rb, so its
overexpression might cause the same effect as overexpression of cyclin D. E. In complementation test most of the phage produced must be wildtype. False: in a mutant by mutant test of complementation (I forgot to say mutant by mutant) most
progeny would be mutant because both genomes can be duplicated if they are
complementing each other. F. In the experiment shown below,the order of genes is not interpretable from the data
shown. The cross was Hfr A+ B+ C+ x F- a- b- c- (read part G if something confuses you). Gene C+ , , ,
% gene Gene A + False, gene order 15 Interpretable from the tIme of
transfer Gene 3+ gene transfer on X aXIs...Is A, B and C In that order. 0 10min 20 min 30 min
G. One aspect ofthe results In F makes absolutely no sense. True--that GeneC+ has a higher level of transfer than A+ or 3+ is nonsensical...since it is further
from the site of transfer, it should achieve an overall lower level of transfer. ...
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- Fall '07