Bio 208 Exam 3 Learning Objectives

Bio 208 Exam 3 Learning Objectives - Exam 3 Learning...

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Exam 3 Learning Objectives CHAPTER 6 – Microbial Nutrition and Growth By the end of this chapter you should be able to: • Discuss the classification of bacteria into groups based on their chemical and physical requirements for growth (temperature, pH, osmotic pressure, oxygen, carbon source, etc). • Describe the methodologies used to culture, isolate, and quantify microorganisms. • Compare and contrast a chemically defined medium with a complex medium. • Discuss, giving examples, how selective and differential media can be used to identify microorganisms. • Distinguish between exponential and linear population growth. • Successfully complete population growth problems. • Draw a graph from a set of population data. Use the graph to identify linear and exponential population growth. • Solve sample problems involving dilution factors. • Describe accepted procedures for enumerating bacteria found in water. Use and interpret a Most Probable Number chart. CHAPTER 9 – Controlling Microbial Growth in the Environment & CHAPTER 10 – Controlling Microbial growth in the Body By the end of these chapters you should be able to: • Describe the basic environmental and physiological parameters, which affect the growth of microorganisms. • Distinguish between bactericide and bacteriostasis. • Cite the factors that influence the effectiveness of anti-microbial treatments. • Identify procaryotic cell structures that are potential targets for anti-microbial control agents. • List physical methods of microbial control. Give an example of each. Describe the effectiveness of each method, mechanism of action, and preferred use. • Differentiate between disinfectant and antiseptic. Give an example of each. • Describe the effects of antiseptics and disinfectants on bacterial growth. • Demonstrate an understanding of the use of the Filter Paper Method and the Use-Dilution Test for determining the effectiveness of an antiseptic or disinfectant. • Cite an example of each of the following chemical disinfectants (phenols, halogens, biguanides, alcohols, heavy metals, surface-active agents, quats, aldehydes, organic acids, and gaseous chemosterilizers). Describe the effectiveness of each chemical, the mechanism of action, and preferred use. • Describe the effects of antibiotics on bacterial growth. • Describe the mode of action of selected antibiotics (penicillins, streptomycin, tetracyclines, sulfanilamide, rifampin, bacitracin, neomycin, trimethoprim and Polymyxin B). • Demonstrate an understanding of the use of the disk-diffusion method for determining antibiotic effectiveness. • Interpret the results of broth dilution tests (determine MIC and MBC values). • List the ideal properties for an antibiotic and describe the significance of each property.
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This note was uploaded on 03/18/2010 for the course BIO 208 taught by Professor Beattie during the Fall '06 term at Kentucky.

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Bio 208 Exam 3 Learning Objectives - Exam 3 Learning...

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