Exam 3 Learning Objectives
CHAPTER 6 – Microbial Nutrition and Growth
By the end of this chapter you should be able to:
• Discuss the classification of bacteria into groups based on their chemical and physical requirements
for growth (temperature, pH, osmotic pressure, oxygen, carbon source, etc).
• Describe the methodologies used to culture, isolate, and quantify microorganisms.
• Compare and contrast a chemically defined medium with a complex medium.
• Discuss, giving examples, how selective and differential media can be used to identify
• Distinguish between exponential and linear population growth.
• Successfully complete population growth problems.
• Draw a graph from a set of population data. Use the graph to identify linear and exponential
• Solve sample problems involving dilution factors.
• Describe accepted procedures for enumerating bacteria found in water. Use and interpret a Most
Probable Number chart.
CHAPTER 9 – Controlling Microbial Growth in the Environment
CHAPTER 10 – Controlling Microbial growth in the Body
By the end of these chapters you should be able to:
• Describe the basic environmental and physiological parameters, which affect the growth of
• Distinguish between bactericide and bacteriostasis.
• Cite the factors that influence the effectiveness of anti-microbial treatments.
• Identify procaryotic cell structures that are potential targets for anti-microbial control agents.
• List physical methods of microbial control. Give an example of each. Describe the effectiveness of
each method, mechanism of action, and preferred use.
• Differentiate between disinfectant and antiseptic. Give an example of each.
• Describe the effects of antiseptics and disinfectants on bacterial growth.
• Demonstrate an understanding of the use of the Filter Paper Method and the Use-Dilution Test for
determining the effectiveness of an antiseptic or disinfectant.
• Cite an example of each of the following chemical disinfectants (phenols, halogens, biguanides,
alcohols, heavy metals, surface-active agents, quats, aldehydes, organic acids, and gaseous
chemosterilizers). Describe the effectiveness of each chemical, the mechanism of action, and
• Describe the effects of antibiotics on bacterial growth.
• Describe the mode of action of selected antibiotics (penicillins, streptomycin, tetracyclines,
sulfanilamide, rifampin, bacitracin, neomycin, trimethoprim and Polymyxin B).
• Demonstrate an understanding of the use of the disk-diffusion method for determining antibiotic
• Interpret the results of broth dilution tests (determine MIC and MBC values).
• List the ideal properties for an antibiotic and describe the significance of each property.