CH204 Experiment 7a - Experiment 7. Synthesis and Analysis...

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1 Experiment 7a. Synthesis and Analysis of Complex Iron Compound. Part 3 of Experiment 5 The Standard Curve Experiment 7. Synthesis and Analysis of Complex Iron Compound. PURPOSE AND LEARNING OBJECTIVES r To use visible spectrophotometry to determine the iron content of a prepared sample s TODAY: To determine molar absorptivity of Fe(phen) 3 2+ from a set of standard solutions. s You will complete parts 2 and 3 today. s Next week, you will complete parts 1 and 4. K 3 [ Fe (C 2 O 4 ) 3 ]· 3H 2 O Iron content Absorption of light Visible Spectrum 400 500 600 700 Wavelength λ (nm) Energy b Any substance that absorbs visible light appears colored to human eye when white light is transmitted through it or reflected from it. b White light contains all the colors in the visible spectrum. λ hc E = Experiment 7. Synthesis and Analysis of Complex Iron Compound. Example: Chlorophyll – absorbs violet and red and appears green b When substance absorbs certain wavelengths (colors) of white light, human eye detects the wavelengths (colors) that are not absorbed (are transmitted). Wavelength range – 325 to 1100 nm Wavelength accuracy – ± 2.0 nm Wavelength precision – ± 0.5 nm λ selector Light source Sample Light detector Readout Amplifie r b An instrument known as a spectrophotometer can detect differences in color intensity, which eliminates human error. b
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CH204 Experiment 7a - Experiment 7. Synthesis and Analysis...

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