Ch7 - Chapter 7 Genetic Analysis in Molecular Biology...

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Chapter 7 Genetic Analysis in Molecular Biology
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Genetic recombination involves exchange between two chromosomes Figure 7.1
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Recombination frequency can be used to determine gene arrangement Recombination frequency is proportional to distance between genes Crossovers take place at random Figure 7.2
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The cell envelopes of gram-negative and gram-positive bacteria differ Figure 7.4
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Bacteria can be grown easily and rapidly E. coli divides every 30 to 60 minutes bacteria can be grown in liquid medium or solid medium broth medium contains a complex extract of biological material minimal medium chemically defined mixture with no organic compounds other than a carbon source
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A typical bacterial growth curve has three phases Figure 7.5
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Bacteria growing on plates form colonies Figure 7.6 Photo courtesy of Dr. Jim Feeley/CDC
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Bacterial auxotrophies can be determined by plating experiments This experiment shows the bacterium is auxotrophic for both Ala and His Data from Freifelder, D. Molecular Biology. Jones and Bartlett Publishers, 1987.
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Conventions used in bacterial genetics Genes and genotypes are indicated in lowercase in italics if there is more than one gene with the same three letter designation, the different genes also have a capital A, B, C, etc (e.g., leuA , leuB ) Phenotypes are indicated with regular type and the first letter is uppercase a superscript + or - denotes the presence or absence of the designated characteristic S and R denote sensitivity and resistance
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Wild type versus mutant the functional form of a gene is usually considered to be the wild type a nonfunctional form of a gene (at least under some conditions) is usually considered to be a mutant both the functional and nonfunctional forms of a gene are alleles
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Classification of mutants absolute defective mutant displays the mutant phenotype under all conditions conditional mutant does not always show the mutant phenotype temperature-sensitive mutant a conditional mutant that behaves normally at the lower permissive temperature but as a mutant at the higher nonpermissive temperature suppressor-sensitive mutant a conditional mutant that exhibits the mutant phenotype in some strains but not others
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This note was uploaded on 03/17/2010 for the course ECON 388 taught by Professor Mcdonald,j during the Spring '08 term at BYU.

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Ch7 - Chapter 7 Genetic Analysis in Molecular Biology...

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